Isocratic elution

Isocratic elution is a method used in chromatography, a process for separating substances in a mixture. In this method, the same solvent or mixture of solvents is used throughout the entire separation process. This constant composition allows for predictable and consistent analysis of the mixture’s components. It is ideal when the substances being separated have similar affinities for the stationary and mobile phases, resulting in a stable environment that simplifies the interpretation of results. Essentially, isocratic elution maintains a steady flow of the same solvent to effectively separate and analyze components without changing the conditions during the process.