Inverted Phase Contrast Microscopy

Inverted phase contrast microscopy is a technique used to observe living cells and organisms without staining them. Unlike traditional microscopes, it has the light source and condenser beneath the sample, which allows scientists to view cells growing in containers like petri dishes. The microscope uses special lenses and lighting to enhance the contrast between different parts of the transparent cells, making internal structures visible without killing or altering them. This approach is especially useful for studying live cells in their natural environment, providing clear images while maintaining cell health.