iDISCO

iDISCO (immunolabeling-enabled three-dimensional imaging of solvent-cleared organs) is an advanced technique used by scientists to visualize and study the detailed structures within large biological tissues, like brains or organs. It involves making tissues transparent through chemical treatments, so light can penetrate deeply. Researchers then use special antibodies to label specific proteins or cells, allowing them to see these components within the whole tissue using powerful microscopes. This method provides a comprehensive, 3D view of complex biological systems, aiding in understanding their organization and function without needing to slice them into thin pieces.