HPLC method development

HPLC (High-Performance Liquid Chromatography) method development involves designing a process to accurately separate, identify, and quantify components in a mixture. It requires selecting optimal conditions—such as the type of solvent (mobile phase), how fast it flows, the nature of the column, and temperature—to achieve clear separation of all substances. This process involves testing different parameters, fine-tuning them to improve resolution, reduce analysis time, and ensure reproducibility. The goal is to develop a reliable, sensitive method that consistently produces accurate results for quality control, research, or regulatory purposes.