Gradient elution

Gradient elution is a technique used in chromatography to separate compounds more efficiently. It involves gradually changing the composition of the solvent (or mobile phase) during the analysis, typically increasing the amount of a stronger solvent over time. This approach helps to first move out the less sticky compounds gently, then more tightly bound ones more quickly, resulting in better separation and faster analysis. Essentially, by dynamically adjusting the solvent's strength, gradient elution improves the resolution and efficiency of separating complex mixtures.