Gateway cloning

Gateway cloning is a technique used in molecular biology to transfer DNA fragments into different vectors efficiently and accurately. It uses specialized enzymes called recombinases that recognize specific DNA sequences, allowing the DNA of interest to be inserted into a vector (such as a plasmid) without the need for traditional restriction enzyme cutting and ligation. This method streamlines the process of cloning genes, enabling rapid and reliable transfer of genetic material for research or biotechnology applications. Essentially, Gateway cloning simplifies the process of moving DNA pieces between different DNA tools while maintaining their integrity.