Fluorometric assays

Fluorometric assays are laboratory techniques that measure how substances emit light when exposed to a specific wavelength of energy, usually ultraviolet or blue light. These assays are used to detect and quantify the presence of particular molecules, like enzymes, DNA, or proteins. When the target molecule is present, it triggers a reaction that produces a fluorescent signal. The intensity of this glow correlates with the amount of the substance in the sample, allowing scientists to analyze biological processes or diagnose conditions accurately. Essentially, it’s a sensitive method to “light up” and measure specific molecules in complex samples.