Fluorescence lifetime imaging microscopy (FLIM)

Fluorescence Lifetime Imaging Microscopy (FLIM) is an advanced imaging technique that measures how long fluorescent molecules emit light after being excited by a laser or another light source. Instead of just capturing how bright a sample is, FLIM records the precise duration of fluorescence, which can reveal detailed information about the molecules' environment, such as pH, ion concentration, or binding interactions. This method produces images that highlight these subtle differences, helping scientists study biological processes, detect diseases, or analyze cellular functions with high sensitivity and specificity.