Fluorescence lifetime imaging

Fluorescence lifetime imaging (FLIM) is a technique that measures how long a fluorescent molecule emits light after being excited by a light source. Instead of just capturing the brightness, FLIM analyzes the decay time of the fluorescence signal, providing information about the local environment of the molecules—such as pH, ion concentration, or interactions with other molecules. This method produces detailed images based on these decay rates, helping scientists and doctors study biological processes, diagnose diseases, and observe cellular functions with high sensitivity and specificity.