Epi-fluorescence microscopy

Epi-fluorescence microscopy is a technique used to visualize specific parts of a sample by using special dyes called fluorophores. The microscope shines a specific wavelength of light onto the sample, exciting the fluorophores, which then emit a different, visible light. This emitted light is detected to create a detailed image of the targeted structures within the sample. The "epi" part means the same lens is used for both illuminating and viewing the sample. This method allows scientists to study the precise location and behavior of molecules inside cells with high specificity and contrast.