dye-binding assay

A dye-binding assay is a laboratory method used to measure the amount of a specific substance, such as a protein or enzyme, in a sample. It works by adding a special dye that binds selectively to the target molecule. When the dye attaches, it changes color proportionally to the amount present. By measuring the color change with a spectrophotometer, scientists can determine how much of the substance is in the sample accurately and efficiently. This technique is widely used because it is straightforward, sensitive, and allows for rapid analysis of biological materials.