CRISPR-based Gene Regulation

CRISPR-based gene regulation uses a specialized tool derived from a bacterial immune system to control gene activity. It involves a DNA-targeting molecule called guide RNA, which directs a protein called Cas9 to specific gene regions. By modifying Cas9 so it no longer cuts DNA but instead influences gene expression, scientists can turn genes on or off precisely. This method allows for detailed study of gene functions and holds potential for treating genetic disorders, enabling targeted and reversible control of genes without permanently altering DNA sequence.