ChIP-seq (Chromatin Immunoprecipitation Sequencing)

ChIP-seq (Chromatin Immunoprecipitation Sequencing) is a technique used to identify where specific proteins bind to DNA in the genome. It involves covering cells to lock proteins onto DNA, then using specialized antibodies to pull out DNA pieces attached to a target protein. These DNA fragments are purified and sequenced to determine their precise locations. This method helps scientists understand gene regulation by showing how proteins such as transcription factors or histones interact with DNA, revealing patterns of gene activity and how genes are turned on or off in different cell types or conditions.