Broth Microdilution Method

The Broth Microdilution Method is a laboratory technique used to determine the smallest amount of an antibiotic needed to stop bacterial growth, called the Minimum Inhibitory Concentration (MIC). It involves placing tiny amounts of antibiotics into small wells of a tray containing a bacterial sample in a nutrient-rich liquid called broth. After incubation, scientists observe which wells show bacterial growth and which do not. The lowest antibiotic concentration that prevents visible bacteria growth is recorded as the MIC, helping to identify effective antibiotics for treating infections.