Broth Dilution Test

The broth dilution test is a method used to determine the lowest concentration of an antibiotic that can effectively stop bacterial growth. It involves diluting the antibiotic in a liquid nutrient solution called broth, then adding bacteria to each dilution. After incubation, scientists observe which samples show visible bacterial growth. The smallest amount of antibiotic that prevents growth indicates the bacteria's susceptibility level. This test helps doctors choose the most effective antibiotic dosage to treat infections while minimizing unnecessary use.