Bicinchoninic acid (BCA) assay

The Bicinchoninic Acid (BCA) assay is a laboratory method used to measure protein concentration in a sample. It works by mixing the sample with a reagent that contains bicinchoninic acid, which reacts with proteins in the presence of copper ions, producing a color change. The intensity of the color formed is proportional to the amount of protein present. By comparing the color to a standard curve created with known protein concentrations, scientists can accurately determine how much protein is in the sample. This assay is widely used in biochemistry and molecular biology for its sensitivity and reliability.