Affinity chromatography

Affinity chromatography is a laboratory technique used to separate specific molecules from a mixture based on their unique binding properties. It involves attaching a special molecule, called a ligand, to a solid material like a bead within a column. When a mixture passes through, only the target molecule binds to the ligand due to their specific interaction, while others flow away. Afterwards, conditions are changed to release the target molecule, allowing it to be collected in a purified form. This method is highly effective for isolating specific proteins, enzymes, or other biomolecules with high precision.