A=εcl (Absorbance Equation)

The equation A = εcl explains how much light a substance absorbs. Here, A is the absorbance, measuring how much light is blocked. ε (epsilon) is a constant unique to each material, indicating how strongly it absorbs light at a specific wavelength. c is the concentration of the substance—the amount present in a given volume—and l is the path length, how long the light travels through the sample. Essentially, the more concentrated the substance and the thicker the sample, the more light it absorbs, resulting in higher absorbance values. This helps scientists determine the concentration of a substance in a solution.