2D chromatography

Two-dimensional chromatography is an advanced analytical technique used to separate complex mixtures of chemical substances. It involves passing a sample through two different chromatography processes in sequence, each with distinct separation principles. The first stage separates components based on one property (like size or polarity), and the second stage separates them by a different property. This dual approach provides higher resolution and better identification of individual compounds, especially in complex mixtures. It’s commonly used in fields like environmental analysis, pharmaceuticals, and forensic science to analyze substances with many similar components, offering more detailed and accurate results than single-step methods.